association study between polymorphisms in mia3, smad3, sele, cetp, lipg and trnathr genes, and coronary artery disease (cad)

Background and aim: one of the main reasons of mortality in the world is cardiovascular diseases, and coronary heart disease (cad) is the most common heart disease that occurs as a result of coronary artery stenosis and atheroma plaque formation. according to the definition of world health organization, cad refers to cases with ≥50% luminal stenosis of any coronary vessel. cad is a multifactorial disease which genetic and environmental factors are involved in it. several studies has shown association between some polymorphism in different genes with cad. finding this association can be used in order to early diagnosis and prevention of cad.methods: 100 cad patients with ≥50% luminal stenosis of any coronary vessel as case group and 100 healthy individuals as control group were selected. after dna extraction by a standard extraction kit, the polymorphisms were evaluated by arms-pcr and pcr-rfp methods. sanger sequencing method was used to confirm the findings. in this study association between rs17228212 in smad3 gene, rs17465637 in mia3 gene,rs3917406, rs1805193 and rs5355 in sele gene, rs5882 and rs708272 in cetp gene, rs2000813 in lipg gene, rs193303002 in trnathr and the risk of cad were evaluated.spss was used for statistical analysis. frequency of different genotypes of the polymorphism was analyzed using chi-squared (χ2) test.results: the results of this study show that there is no significant association between rs17228212, rs17465637, rs193303002, rs1805193, rs708272 and rs2000813 and risk of cad in the studied patients. but there is significant association between rs5355 (p=0.03), rs3917406 (p=0.02), and rs5882 (p=0.007), and risk of cad.conclusion: our results, according to previous studies confirmed although there was no direct association of cetp activity with cetp polymorphisms, our findings revealed a significant interaction between cetp snps and cetp activity for affecting hdl-c levels. the sele gene is a known cell adhesion molecule with a significant role in inflammation. mounting evidence has suggested inflammation as an important component in the pathological process of coronary atherosclerosis. studies about possible linkage between sele gene polymorphisms and the development of cad are conflicting. we have found a significant association between polymorphisms of sele gene and risk of cad.