Competition of Mir-451 in Breast Cancer Using Rt-Qpcr

Background and aim: breast cancer is the most common invasive cancer in females worldwide. it accounts for 16% of all female cancers and 22.9% of invasive cancers in women. mir-451 is the most down-regulated mirnas in inflammatory breast carcinoma and able to enhance tamoxifen sensitive. the aim of this study was to compare the expression level of mir-451 in normal and neoplastic samples from breast cancer patients using rt-qpcr.methods: in the present case-control study, 20 samples from patients with breast cancer tumor and 20 patients from non-tumor were collected under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings. after extracting rna from normal and tumor tissues, cdna synthesis method according to the protocol and rt-qpcr were performed by sybr®premix ex taqtm ii kit. mir-451 expression levels was calculated using ∆∆ct. data were analyzed using t-test. results: the results of molecular method indicated that the mean relative expression levels of mir-451 in tumor samples compared to normal was down-regulated. this variation expression of mir-451 in tumor samples was about 2.1 fold less than normal samples. conclusion: according to previous reports, mir-451 may play an important role in breast cancer progression, and that the down-regulation of mir-451 may serve as independent predictors for breast cancer.