Down-Regulation of Survivin Involved in Copper (Ii) Phenylthiosemicarbazone Complex-Induced Apoptosis in Leukemia Stem-Like Kg1a Cells

Background and aim: previous studies suggested that phenylthiosemicarbazones considered as a new apoptosis-inducing agent. in this study, anti-proliferative and apoptotic effects of the copper (ii) phenylthiosemicarbazone complex (cu-ptsc) were investigated in human acute myeloid leukemia kg1a cell line. the kg1a cells were treated with various concentrations of the cu-ptsc (20-140 μm), and cell viability was determined by mtt assay.western blotting showed that survivin expression considerably down-regulated 24-72 h after treatment with an ic50 concentration of the cu-ptsc. the cytotoxicity of an active compound from copper (ii) phenylthiosemicarbazones (cu-ptsc) was investigated against kg1a cells a model for leukemic stem cell in aml. we were interested in understanding its cytotoxic effect and underlying mechanisms of action of the compound on kg1a cells. this compound caused a significant decrease in the kg1a cell viability in a dose and time-dependent manner.methods: cell culture western blotting analysisresults: cu-ptsc induced apoptosis in kg1a cells cu-ptsc inhibited kg1a cell viabilityconclusion: based on present data, it seems that cu-ptsc may provide a novel therapeutic approach for the treatment of acute myeloid leukemia