In Silico Identification and Annotation of Los2 Gene in Walnut (Juglans Regia)

Background and aim: low temperature stress is one of the biggest environmental factors that limit the growth, development, productivity, and distribution of agricultural crops in the world. cold tolerance is the major method by which plants adapt to stress through the regulation of a cascade of cold-responsive genes. a wide network of different genes is involved in the regulation of tolerance to this stress in plants. los2 represses the transcription of sts/zat10 (a negative regulator of cold responsive genes).methods: in this study, the amino acid sequence of this gene from arabidopsis thaliana (as a model plant) was used as a query to search against the walnut genome database using blastp tool. for the prediction, compare and annotation of obtained sequences, various tools (such as; cd-search (conserved domains), protscale(transmembrane regions), psipred(secondary structure), cobalt(phylogenetic tree) and swiss-model(three dimensional structure)) were used for analysis. results: the results showed that the similarity of the amino acid encoding sequence was 88%. the conserved domains, transmembrane regions, secondary and three dimensional structures prediction results showed that the amino acid sequence of both of sequences had a similar structure. conclusion: by analyzing the walnut los2 and its encoded gene bioinformatics features, we can provide the theoretical reference for the los2 gene cloning and the genetic manipulation.