Molecular Diagnostics of Candida Albicans -Secreted Aspartyl Proteinase Genes From Women’S Urinary Tract Infections Yeast Agent in Sari City

Background and aim: candidiasis is the most common fungal infectious disease. candida albicans is the most important pathogenic candida strain that can cause severe urinary tract infections in women. c. albicans secreted aspartyl proteinases (sap) encoded by a family of 10 genes. the function of sap proteinases is important during infection and enhances the pathogenic ability of c. albicans. the aim of the present study were, molecular diagnosis of c. albicans genes on urinary tract infections agent in women in sari city.methods: fifty of c. albicans isolates were obtained from patients with vaginitis symptoms who had referred to obstetrics and gynecology clinic in sari city. dna extraction was performed using a dna extraction kit and amplified by pcr to detect sap genes with specific primers that were complementary to the c. albicans acting gene. the pcr product was sequenced and fasta format of sequences were extracted using chromas lite software. after the determination of sequences, sequence analysis was performed by blast program in ncbi database. dna sequences translated to amino acid sequences using sequence translation tools (ebi, (expasy server). then, the three-dimensional structure and the secondary and estimated protein structure were determined by phyer2 protein fold recognition server.results: results of homology comparison in the genbank database using ncbi/blast shows c. albicans gene sap1 were 97% homologous with c. albicans gene sap1 from brazil (bartelli et al., 2017). the c. albicans gene sap1 nucleotide sequence (658 nucleotides) translated to 220 amino acids sequences. secondary structure of protein of c. albicans gene sap1 contains 29% alpha helix and 26% beta sheet structure. results of homology comparison c. albicans gene sap3 shows 97% homology similarity with c. albicans gene sap3 complete sequences from usa (white et al., 2008). the c. albicans gene sap3 nucleotide sequences (664 nucleotides) translated to 222 amino acids sequences.conclusion: conclusion of homology comparison c. albicans gene sap6 shows 99% homology similarity with c. albicans sap6 gene for aspartyl protease from switzerland ((monod et al., 1994). c. albicans gene sap8 were 94.73% homologous with c. albicans gene sap8 from india (rajpara, et al., 2018).