Knockdown of Long Non Coding Rna of Link-A Suggests A Potential Oncogenic Function in A549 Non-Small Cell Lung Carcinoma

Background and aim: long non coding rnas (lncrna) play an important regulatory roles in various pathways which dysregulation of them impact on cancer. link-a (long intergenic noncoding rna for kinase activation) is an oncogenic cytoplasmic noncoding rna with approximately 1.5 kbp length. link-a is involved in a pathway, which ultimately leads to hyperactivation of hif1α in normoxic condition in triple negative breast cancer. oncogenic function of link-a was indicated in ovarian cancer and in human glioma cells, but its function in most of cancers is still unclear. in this study we were going to functionalize link-a in a549 cell line as a model of non-small cell lung cancer.methods: expression of link-a was detected in a549 cell line by rt-pcr and confirmed by cloning in ta-vector and sequencing. two specific sirnas were used to downregulation of link-a, along the experiment, we used hs cell death control sirna as a positive control and scrambled sirna as a negative control. consequences of link-a downregulation was examined by mtt, colony formation, and wound healing assay and also cell cycle analysis, annexin v/pi, acridin orange and ethidium bromide staining were conducted to examine occurrence of apoptosis. qpcr was used to determine rna expression change of link-a and some other genes downstream of hif1.results: our results revealed reduced cell viability and colony formation ability influenced by link-a silencing. we also observed the induction of apoptosis and declined 2-d cell mobility, following by link-a downregulation.conclusion: our findings suggest a potential oncogenic role for link-a in a549 cell line as a representative for non-small cell lung cancer for the first time.