non-invasive detection of prostate cancer using mir-25 expression level in patients urine in compared with normals

Background and aim: prostate cancer is the most common cancer in men and has a significant mortality rate. diagnosis of prostate cancer is more based on aggressive methods such as biopsy. finding new biomarkers that can lead to non-invasive and early diagnosis of prostate cancer is of interest. in this study indicate that selected mirna (mir-25) may promising in human cancer early diagnosis.methods: 40 patients enrolled in this study. the case group consists of 30 patients (12 metastatic and 18 non-metastatic) and control group consists of 10 healthy controls. urine and blood samples were obtained. serum psa evaluated and after extraction of total rna and then cdna synthesis, expression of mir-25evaluated in urine specimens.results: the results revealed that in cancer patient group, mir-25 showed a significant reduction in expression in comparison with healthy controls (p=0.001). by using roc curve analysis, specificity, sensitivity were obtained for mir-25, 83% and 61/86%.conclusion: in general, it can be concluded mir-25 have a significant change in expression in patients with prostate cancer compared to healthy controls.a combination panel of mir-25 and other specific mirnas can be used as a non-invasive method for detecting prostate cancer.