Aberrant Expression of Mir-4516 in Breast Cancer Tissue and Its Correlation With Clinicopathological Factors

Background and aim: mirnas are small noncoding rna with 20-25nt in length that known to be aberrantly expressed in various diseases, including cancer. mir-4516 dysregulation was observed in various cancers; its overexpression has been reported in papillary thyroid and prostate cancer. in addition, reduced mir-4516 expression inhibits tumor growth and induces apoptosis in glioblastoma. unlike the other cancers, still there is no information available on mir-4516 in breast cancer. therefore, in this study we evaluated mir-4516 expression and association with clinopathological factors in breast cancer.methods: in this study 35 paraffin-embedded breast cancer tumor tissues with adjacent healthy tissue were enrolled. the total rna was extracted with mn kit (nucleospin® totalrna ffpe- germany). the mirna cdna was synthesized in polyadenylation method. real-time pcr was used to investigate mir-4516 expression. u6 was selected as internal reference gene for normalization of real-time rt-pcr. relative gene expression was analyzed by the 2−δδct method. eventually we analyzed data using statistical methods including t-test and one-way anova.results: according to the analysis, mir-4516 expression was significantly downregulated in tumor tissues compared to adjacent nontumor tissues (p = 0.001). in addition significant decrease in expression of mir-4516 was observed in grade 2 tumors (p = 0.003).conclusion: taken together, these findings indicate that mir-4516 may act as tumor suppressor in breast cancer. in addition, mir-4516 expression is associated with high grade of breast cancer.