Microrna-143 Inhibits Metastasis of Mda-231 Breast Cancer Cell Line

Background and aim: breast cancer (bc) is the most common cancer among females worldwide and the second cause of mortality associated with tumors after lung cancer. according to global cancer statistics, approximately 2.1 million newly diagnosed cases of breast cancer in women will rise globally in 2018, accounting for about 1 in 4 cases of cancer in women. given the advances in cancer therapy, this malignancy nevertheless appears incurable owing to metastasis and medication resistance. nonetheless, studying the bc tumorigenesis process could be an effective way to overcome this issue. over the past decades, the biology of micrornas (a type of small non-coding rna) has grown tremendously as potential therapeutic targets in cancer. mir-143 is down-regulated in a wide range of human cancers including bc as a tumor suppressor mirna and plays an important role in cell proliferation and metastasis. in various cancer cell lines, overexpression of mir-143 inhibits cell proliferation, indicating that the absence of mir-143 is observing in human cancers survival and amplifies tumor growth. then mir-143 replacement could be an effective therapeutic strategy.methods: mda-231 cell lines purchased from pasture institute were cultivated in rpmi medium. in order to cell migration assay, mda-231 cells treated with mir-143 mimics and they were seeded in 24-well plate. after 12 hours, we scratched the wells and the scratched area was quantified for 24 hours. on the other hand in order to evaluate the expression levels of metastatic genes, we have assessed the expression level of vimentin, mmp3, and mmp9 by qrt-pcr.results: overexpression of mir-143 in bc cells compared with control cells inhibits metastasis through vimentin, mmp3, and mmp9 down-regulation.conclusion: according to our results, mir-143 replacement through down-regulation of vimentin, mmp3, and mmp9 may inhibit bc cell metastasis and be considered as a potential therapeutic strategy in bc treatment.