Study on Genetic Diversity of Iranian Apple Cultivars By Ssr Markers

Background and aim: in order to study the genetic diversity of iranian apple cultivars, a experiment was preformed with 13 apple genotypes and overall 43 sample at department of genetics and national plant gene bank of iran (npgbi).methods: samples plant from gardens and research stations of kamal abad, karaj & khorasan was collected. in this study after extracted dna and determine the quantity and quality of that, 10 primer microsatellite (ssr) on poly acrylamide gel were evaluated, and of these, hi21f08, hi03a03, ch04g10, hi07h02 and hi01c11 primers showed most polymorphs. band pattern observed for each primer based on bands there, 1 and absent 0, it was considered and calculated polymorphism information content (pic) and shannon index. also cluster analysis obtained based upgma algorithm.results: the results showed; 25 polymorphic alleles were proliferated in 5 microsatellite loci. genetic diversity indices showed that average of polymorphism information content (pic) was 31% that hi21f08 and hi01c11 had the highest (0.37) and lowest (0.17) values of polymorphism information content respectively and ch04g10 locus had the highest rate (1.55) of shannon diversity index, which represents the genetic diversity among populations, whereas hi01c11 had the lowest (1.00) shannon index. cluster analysis can be divided 13 apple genotypes of iran into seven groups,conclusion: this indicates that high genetic diversity is between apple cultivars of iran.