Pax8 Gene Expression Analysis in Thyroid Neoplasm

Background and aim: thyroid neoplasm is the most common endocrine cancer in the world and its growing incidence has become a worldwide issue. pax8 gene regulates thyroid specific genes and also genes with important roles in the thyroid follicular cells function. the aim of this study is to evaluate the potential of pax8 gene for the diagnosis of the thyroid neoplasm.methods: thyroid neoplastic and their neighboring healthy tissues were collected from sina hospital, isfahan, iran. rna extraction, cdna synthesis and rt-qpcr reaction were performed using specific exon-junctioned primers. beacon v8.21 software and oligo v7.0 software were used for specific primers designing and confirmation. rest 2009 software would be used for data analysis.results: pax8 gene (ncbi-id: 7849) analysis by string database revealed the potential role of the selected gene in thyroid homeostasis. kegg databases also showed that pax8 could cause thyroid cancer development. the designed primers had specific binding only to pax8 gene, appropriate 3’?g and they lacked secondary structures such as hairpins. agarose gel electrophoresis (1%) showed the acceptable quality of the extracted rna. the a260/a280 ratio was 1.8-2.2 that indicate the purity of the extracted rna.conclusion: pax8 gene as an important regulator of vital functions such as transcriptional activity, cell proliferation and apoptosis, plays important roles during thyroid tumorigenesis. some studies have been shown that pax8 expression was decreased in follicular carcinoma. in addition the pax8–pparγ fusion oncogene is common in follicular thyroid cancer but not in other type of thyroid neoplasms. it must be mentioned that the pattern of pax8 expression is still unclear. therefore in this study we would investigate the amount of pax8 expression in cancerous and neighboring healthy tissues to determine the role of pax8 in thyroid tumorigenesis and cancer development.