Identification of Molecular Markers Associated With Seed Vigour Index of Rice Using Recombinant Inbred Line Population

Background and aim: seed germination is one of the most critical stages in the growth and development of the plant. identification of important genomic regions involved in the control of germination components is a main and basic step in breeding programs. seed vigor as a germination component is an important index of seed quality and a component of seed physiological potential. recombinant inbred lines (rils) are powerful tools for genetic mapping.methods: this present study aimed to investigate the seed vigour index of rice among 154 recombinant inbred lines and the identification of microsatellite and est-ssr markers associated with this trait. the experiment was conducted using recombination inbred line population derived cross between shah-pasand and ir28 at university of guilan in 2019. in this regard, 110 ssr and est-ssr markers were assessed on parents of population and identified 41 polymorphic markers.results: five informative markers including rm16030 (on chr.3), rm452 (on chr.2), rm12146 (on chr. 1), rmes6-1 (on chr.6) and rm10890 (on chr. 1) had a significant association with seed vigour index and explained 14.3, 9.9, 9.8, 6.6 and 5.3% of phenotype variation respectively.conclusion: the highest coefficient of determination was estimated for rm16030. the results of the study after validation can be utilized to improve the seed vigour of rice in the breeding program.