Anticancer Effect of Gemini Nanocurcumin in Colon Cancer Cell Lines

Background and aim: colorectal cancer is the third frequently diagnosed illness in the world. curcumin is polyphenolic agent in turmeric plant, which has been demonstrated to have a strong anticancer effects. yet, its low solubility and poor uptake restricts its application. we aimed to study the anticancer activity of gemini nanocurcumin (gnc) in colon cancer cell lines. methods: the toxic effects of gnc on p53 mutant/wild-type colon cancer cell lines including ht-29 and hct116 was studied through mtt, uptake kinetics, fluorescence microscopy, annexin v/fitc, and cell cycle assays. also, qrt-pcr and western blot were performed to evaluate p53, p21, bax, bcl-2, and noxa expression at both rna and protein levels. results: our data revealed that , gnc increased the subg1 cells and induced g2/m cell cycle arrest. in addition, real-time pcr and western blot assays showed an increased expression of bax, noxa, p21, and p53 in a time/dose-dependent manner and a decreased expression of bcl-2 in desired colon cancer cells. conclusion: our study showed that gnc, can perform its anticancer effects by modifying the expression of apoptotic and proliferative genes in both p53 wild-type and p53 mutant colon cancer cell lines via different effective doses.