Study of Mir-342-3p Expression Profile in Human Breast Cancer

Background and aim: micrornas (mirnas) are small non-coding rnas that contribute to modulating signaling pathways after radiation exposure and have emerged as a potential therapeutic target or biomarker in the radiation response of cancer. mir-342-3p is lower in pre-menopausal inflammatory breast carcinoma patients, associated with er levels. this study was aimed to compare mir-342-3p expression profile in human breast cancer using rt-qpcr. methods: twenty samples from patients with breast cancer and twenty samples from non-tumor were collected by a pathologist specialist. after rna extraction from tumoral and normal tissue samples, cdna was synthesized according to protocol and rt-qpcr carried out using sybr®premix ex taqtm ii kit. mir-342-3p expression profile was analyzed by δδct method. results: our results revealed that the mean of mir-342-3p expression profile in human breast cancer is down-regulated in comparison with control group. conclusion: overall, the results of the present study revealed distinct differences in the expression profile of mir-342-3p to control group (down-regulation of mir-342-3p), and this mirna can be used as biomarkers for cancer therapy.