Conservation of Avian Glucagon Has Break By C.209c>G P.70t>S Mutation Only in Two Separate Avian Orders

Background and aim: albeit avian high blood glucose level is a physiologic exception in animals, there is a spectrum of different blood glucose level in order aves. the aim of this study was comparing amino acid sequence of glucagon as an important hormone in glucose homeostasis.methods: we obtained the sequence of gallus gallus glucagon from ncbi and blast its 29 residue in all available aves. afterwards, we checked birds with this mutation to find their taxonomic information. then their corresponding mrnas were checked and the related mutated nucleotides were distinguished.results: by using this bioinformatics approach we found a break in conservation of glucagon in this class of animals only at p.70t>s position. the birds with mutated glucagon were belongs to three separate orders of galliformes and strigiformes. interestingly, c.209c>g mutation was seen these orders which is responsible for change of genetic code from threonine to serine. this mutation may interprete higher blood glucose level in these to orders.conclusion: therefore, according to occurrence of this mutation in separate orders of birds it can be concluded that this mutation may be considered as a non-lethal adaptive mutation in some avian orders.