anticancer potential of euphorbia tehranica ethyl acetate extract against sw480 colorectal cancer cell line

Background and aim: it has been reported that different species of euphorbia have antitumor activity. euphorbia tehranica is considered a traditional herb which is distributed in iran. the anticancer activity of ethyl acetate extract (eae) from this herb against colorectal cancer is unclear. in this study, we evaluated the growth inhibitory effects of euphorbia tehranica extracts on human colon cancer cell line sw480.methods: the ethyl acetate was extracted from euphorbia tehranica. the sw480 cells were treated with different concentrations of eae (5 to 250 μg/ml) at 24, 48 and 72 hours. cell survival was evaluated using mtt assay. anticancer activity of the eae was assessed by evaluating the cell viability. cell viability was determined at the wavelength of 546 and 630 nm by mtt method. the ic50 was determined graphically.results: in this regard, the eae was found to markedly inhibit the proliferation of sw480 cells in a dose and time-dependent manner. the cell mortality increased when the concentration of eae increase from 5µg/ml to 100µg/ml. eae treatment arrested growth in ic50 concentration range. ic50 for sw480 cells was in 50 µg/ml concentration after 24 and 48 hours and 40 µg/ml after 72 hours.conclusion: we inferred that the eae of euphorbia tehranica could have chemo preventive potential against human colorectal cancer. our results may suggest that the gene expression which is contributed in apoptosis has been changed under pressure of eae.