Bioinformatics Study of Anticancer Activity For Wild and Mutant A19k Brazzein

Background and aim: anticancer peptides can be a useful and valuable factor in treating cancer. brazzein protein can act as an anticancer peptide due to its specific structure and sequence. toll-like receptors (tlrs) are innate immune receptors that are expressed in both of innate immune cells and various cancer cells and play a crucial role in the initiation and progression of cancers. according to previous researches, signaling of a tlr 5 in human breast cancer has a strong anti-tumor activity. methods: in this study, the molecular binding of both wild and mutant (a19k) brazzein with tlr 5 was investigated using the haddock server. results: the results suggest that brazzein could be a good candidate as an anticancer drug. conclusion: according to docking, increasing the positive charge in the residue 19 of brazzein increases its anti-cancer properties and improves the docking parameters compared to its wild type, and it seems that the mutation applied, plays an important role to bindings site of receptor. however, for confirmation, the results of this study require experimental studies.