Investigating Expression Level of Some Genes Involved in Production of Ros and Apoptosis in Sperm Before and After Separation With Macs

Background and aim: infertility is defined as inability to get pregnant after at least one year of unprotected sex pursuant to world health organization (who) definition. according to who reports about 70 million couples suffer from infertility all over the world. prolonged generation of ros leads to oxidative stress which has a negative impact on sperm quality, especially membrane integrity, and apoptosis stimulation. oxidative stress occurs from an imbalance between systemic ros production and the ability to detoxify ros by mechanisms such as antioxidant defenses. however, antioxidants should not completely eliminate ros because some ros are needed for normal reproductive functions. in this study, we have measured expression levels of romo-1, salf, ddit-4, ogg-1, bax, bcl-2, bcl-x genes that are involved in the production of reactive oxygen species in sperms of oligospermia patientsmethods: at the first, sperm samples were obtained from 15 oligospermia patients. after liquefying and initial washing of semen samples, crucial investigations were done like confirmation of oligospermia and characteristic criteria such as sperm morphology and motility. then each sample was divided into two groups. for one group, isolation of normal cells from apoptotic cells was done using a negative selection of the macs technique by targeting annexin v as an apoptotic cell surface marker. after isolation of rnas from sperm samples of two groups, the expression levels of romo-1, salf, ddit-4, ogg-1, bax, bcl-2, bcl-x genes were measured using quantitative real time-pcr.results: the results indicated that expression levels of romo-1, salf, ddit-4, ogg-1, bax, bcl-2, bcl-x genes differ before and after macs. apoptotic genes and ros production genes shows statistically low expression after macs (p<0.05).conclusion: our study indicated that the macs technique separates apoptotic cells and sustains normal cells. using quality and healthy sperms increase the ivf success rate and it seems separation of healthy sperms by the macs technique is more reliable. many studies are needed to isolate healthy sperms by other criteria that may influence ivf success.