Anril Variants Are Associated With Risk of Neuropsychiatric Conditions

Background and aim: the antisense non-coding rna in the ink4 locus (anril) is a long non-coding rna (lncrna) whose contribution in several human disorders has been verified.methods: in the current projects, we genotyped two single nucleotide polymorphisms (snps) in this lncrna (rs1333045 and rs1333048) in a population of iranian patients with bipolar disorder (bp), major depressive disorder (mdd), and methamphetamine addiction.results: the rs1333045 was associated with methamphetamine addiction in recessive and multiplicative models (or (95% ci) = 1.867 (1.211–2.877), adjusted p value = 8.75e−03 and or (95% ci) = 1.415 (1.089– 1.839), adjusted p value = 1.87e−02 respectively). the rs1333048 was associated with methamphetamine addiction in codominant model (a/a vs. c/c: or (95% ci) = 0.195 (0.114–0.336), adjusted p value = 2.44e−09) and in other inheritance models. the rs1333045 was not associated with risk of bp i in any inheritance model. however, the rs1333048 was associated with bp i in co-dominant model (a/a vs. c/c: or (95% ci) = 0.499 (0.286–0.870), adjusted p value = 2.53e−07) and in other inheritance models. in bp ii cohort, we detected significant associations between both snps and risk of disorder in all inheritance models. in co-dominant model, these associations were detected just between homozygotes (t/t vs. c/c (rs1333045); a/a vs. c/c and (rs1333048)). the rs1333045 was associated with mdd in recessive model (or (95% ci) = 2.221 (1.173–4.207), adjusted p value = 0.026). the rs1333048 was associated with mdds in dominant, recessive, and multiplicative models. the selected snps were not in linkage disequilibrium (d′ statistic0.23, r2 = 0.05). haplotype analyses have shown that t a haplotype block (rs1333045 and rs1333048 respectively) significantly decreases risk of addiction, bp i, bp ii, and mdd. besides, the c c haplotype decreases risk of addiction, bp ii and mdd. finally, the t c haplotype increases risk of bp i, bp ii, and mdd.conclusion: based on the above-mentioned data, the selected anril snps or other snps in linkage disequilibrium with them might confer risk of neuropsychiatric disorders. taken together, anril can be regarded as a risk locus for these conditions.