Evaluation of the Expression of Nptn-It1 Long Non-Coding Rna in Breast Cancer and Its Relationship With Clinicopathological Information and Reproductive Factors

Background and aim: breast cancer causes the highest related death among women. long non coding rnas (lncrnas), a subclass of non-coding rnas, which are involved in significant functions in regulation of gene expression. however the tumor suppressive roles of lncrna nptn-it1 has been identified in some cancers, its expression in breast tumors and normal tissues is still unknown. in this study, we aimed to assess the expression of lncrna nptn-it1 in breast tumors and normal tissues, and in these contexts we investigate the potential lncrna nptn-it1 /mirna/mrna axes.methods: the expression of lncrna nptn-it1 was analyzed using real-time rcr. also, we assessed the association of lncrna nptn-it1 expression with demographic and clinicopathological information of patients. then, a bioinformatics approach was used to revealed the critical lncrna nptn-it1 -mediated sponge regulatory network.results: lncrna nptn-it1 expression was downregulated in both breast cancer tissues and adjacent normal tissues. also, it was found that decreased expression of lncrna nptn-it1 is significantly related to early-onset menarche and late-onset menopause. the bioinformatics analyses proving that lncrna nptn-it1 has function as a tumor suppressor sponge.conclusion: together, these results indicate that lncrna nptn-it1 shows a significant down expression in breast cancer tissues and adjacent normal tissues compared with normal mammary tissues. these data suggest that normal breast tissues can undergo tumor-related molecular changes. in silico analyses show that lncrna nptn-it1 plays an essential role in transcriptional misregulation in various malignancies.