The Meaningful Change in Antibiotic-Resistant Gene Expression of Staphylococcus Aureus By Using Functionalized Carbon Nanotubes Nanofluid

Background and aim: staphylococcus aureus is a gram-positive bacterium and has been considered as an important nosocomial pathogen universal owing to its increasing antibiotic resistance and carries the tet (m) gene encoding a tetracycline efflux pump. recently, carbon nanotubes have defined as novel and potential antibacterial and nanocarrier agents. aim: in this investigation, antimicrobial effects study of carbon nanotubes nanofluid on staphylococcus aureus and gene expression study on the tet (m) gene done.methods: cell viability assay was carried out after incubation of staphylococcus aureus by the cnt's suspensions (100 µg /ml) and antibiotic for 24, 48, and 72 h. then the gene expression profile of the tet (m) gene was studied by real-time pcr method on bacteria, which were treated by functionalized carbon nanotubes nano fluid+ antibiotic in comparison with antibiotic alone.results: results were conferred significantly, reduced bacterial proliferation, and reduced expression of the tet (m) gene in the nano fluid+ antibiotic condition.conclusion: the present study showed that the functionalized carbon nanotubes nanofluid could be a proper drug delivery vehicle for overcoming bacterial antibiotic-resistant.