The Significant Change in Antibiotic-Resistant Gene Expression of Acinetobacter Baumannii By Using Functionalized Carbon Nanotubes Nanofluid

Background and aim: acinetobacter baumannii is one of the most critical factors in the development of nosocomial infections around the world and carries the tet (a) gene encoding a tetracycline efflux pump. recently, carbon nanotubes have described as a novel and potential antibacterial and nanocarrier agents. aim: in this research, antimicrobial effects study of carbon nanotubes nanofluid on acinetobacter baumannii and gene expression study on the tet (a) gene was done.methods: cell viability assay was carried out after incubation of acinetobacter baumannii by the cnt's suspensions (100 µg /ml) and antibiotic for 24, 48, and 72 h. then the gene expression profile of the tet (a) gene was studied by real-time pcr method on bacteria, which were treated by functionalized carbon nanotubes nano fluid+ antibiotic in comparison with antibiotic alone.results: results were shown significantly, reduced bacterial proliferation, and reduced expression of the tet (a) gene in the nano fluid+ antibiotic condition.conclusion: the present study showed that the functionalized carbon nanotubes nanofluid could be a suitable drug delivery vehicle for overcoming bacterial antibiotic-resistant.