Investigation of Therapeutic Effects of Stem Cell (Hamsc) on Pancreatic Cancer Cell (Mia Paca2) By Evaluating of Epidermal Growth Factor Receptor (Egfr) Expression Level

Background and aim: mesenchymal stem cells (mscs) are a population of pluripotent stem cells with potential applications in regenerative medicine and tumor targeting therapy. it was found that tumors are under the effects of tumor microenvironment, which consisted of mesenchymal stem cells (mscs), growth factors, cytokine networks, tumor vasculature, and extracellular matrix. the interactions between mscs and tumor cells are complicated, and the mechanisms involved are multifaceted. the study of molecular events associated with msc/tumor cell interactions would be essential for identifying the role of these important stem cells in physiological and pathophysiological conditions. in this study, we investigated the possible effects of hamsc on the pancreatic cancer cell (mia paca2) by using co-culture method. in this regards, we are interested to evaluate the expression level of egfr as one of the most critical molecules to regulate the cell signal transduction pathways.methods: mesenchymal stem cell from human cord blood (hamscs) and pancreatic cancer cell (mia paca2) were prepared and were separately cultured in dulbecco's modified eagle's medium (dmem) medium with 10% fetal bovine serum and in a sterile humidified incubator with 5% co2 at 37˚c. then, these two cell lines were co-cultured in double culture plates for 24 and 72 h. mia paca2 cells were considered the control group and underwent the same culturing time. the rna of treated and untreated cancer cells was extracted and then egfr expression was analyzed by using quantitative real time pcr (qrt-pcr). for further study, total cell lysates of related cells were prepared and the expression of egfr protein was analyzed by using western blot.results: the expression of egfr in pancreatic cancer cell under the co-culture with hamsc was decreased. of note, the results from western blot analysis confirmed qrt-pcr results.conclusion: our results showed that interaction between stem cells with pancreatic cancer cells has inhibitory effects on cancer cells and it can be consider as a therapeutic way to suppress egfr as a potent target of cancer cells signaling. however, more study will be required to find all critical players in this field.