The Effect of Mirna- 4422 Replacement Therapy in Porolifretion, Apoptosis,Metastasis and Migration of Kato Ш Cell Line in Vitro.

Background and aim: gastric cancer is one of the most important gastrointestinal cancers and the leading cause of death. micrornas, as significant cellular regulators by affecting their target genes, are involved in growth, apoptosis, differentiation and cell proliferation.in the present study, mir-4422 was selected as a new class of mirnas discovered for replacement therapy.methods: in this study, the kato ш cell line cultured as a human gastric adenocarcinoma cell. mir-4422 was analyzed by bioinformatics analysis and its target genes and biological pathways were also identified mirna mimic mir-4422 was performed by transfection it into the kato ш cells. eventually, modification in migratory and proliferative potential of the cells were respectively examined by wound healing and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (mtt) assays. furthermore, to recognized apoptosis occurrence in the transfected cells, 4',6‐diamidino‐2‐ phenylindole (dapi) staining was used and annexin-pi assay was used to measure the apoptosis and necrosis by flow cytometry. moreover, the level of expression the target genes of mir-4422 such as mapk8, smad2, smad4, smad8, grb2 and ptpn3, were assessed by quantitative real‐time pcr(qrt‐pcr).results: according to the result, a decreased migratory potential and viability were perceived for the mir-4422 transfecting cells. in addition, mapk8, smad2, smad4, grb2, were significantly downregulated in the transfected cell with mir-4422. however, smad8 and ptpn3 were not significantly downregulated in the transfected group compared to the control group.conclusion: in summary, therapeutic and prognostic modalities so significant in gastric cancer. mir‐ 4422 replacement would be considered as an impressive strategy for the management of gastric cancer and lessening its invasive features.