Quercetin-Induced Downregulation of Lncrna Uca-1 in Mcf-7 Cells

Background and aim: abstract breast cancer is one of the most common malignancies that has serious effects on women´s health. the woman's lifetime risk for developing breast cancer is about 10%. the uca1 gene produces a long non-coding rna that have been diagnosed in various cancers, such as breast cancer. food-derived flavonoid quercetin, has therapeutic potential in cancer, cardiovascular and neurological diseases. the aim of this study was to evaluate the effect of quercetin on the expression of lncrna uca1 in breast cancer cells.methods: the effect of quercetin on the expression of uca1 gene in mcf-7 cells of breast cancer was assessed by treating cells with different concentrations of quercetin at 24h via mtt assay, ic50 was determined; rna was extracted using triazole and then cdna synthesized. the expression levels of uca1 gene relative to gapdh gene at different concentrations of quercetin were quantified using the highly sensitive real time- pcr method.results: the results showed that quercetin has dose-dependent inhibitory effect on the viability of mcf-7 cells. the quantitative real-time polymerase chain reaction results showed that uca1 gene expression was significantly (p=0.001) downregulated in the presence of quercetin.conclusion: quantitative real-time pcr results showed that qurcetin could decrease mcf-7 cell number by decreasing uca1 gene expression.