Viability Assessment of Human Colon and Gastric Adenocarcinoma Cells After Treatment With Nano-Mgo

Background and aim: colon and gastric cancers are major clinical concerns worldwide, whit high incidence in developing and industrial nations. adenocarcinoma of the colon makes more than 90% of all colorectal cancer cases, and develops in cells lining of the large intestine. since mortality rate of colon and gastric adenocarcinomas are high in iran, the aim of present study was to determine effects of nano-mgo synthesized by luminescent vibrio.methods: biosynthesis of nano-mgo was carried out in culture medium containing mg(no3)2/ mgn2o6. after centrifugation, pellets were freeze-dried and dmso was used to dissolve obtained nano-mgo, although its solubility was low. to prepare final dilutions, rpmi or dmem supplemented with 10% fbs were used just before each experiment. for cytotoxicity assessment, human colon and gastric adenocarcinoma cells (ht29 and mkn45 cell lines, respectively) were treated with 5, 10 and 20 µg/ml biosynthetic nano-mgo, as well as dmso alone as relevant control. after 24 h, viability of cells was evaluated by resazurin and morphological alterations of cells were recorded by an inverted microscope.results: evaluation of ht29 cell viability indicated that 95%, 87% and 82% of ht29 cells were alive upon 24 h treatment with 5, 10 and 20 µg/ml nano-mgo, respectively. in contrast, mkn45 cell viability was determined as 100%, 100% and 73%, upon administration of 5, 10 and 20 µg/ml nano-mgo, respectively. taken together, obtained results indicated that nano-mgo induced more toxic effects on mkn45 cells when used in concentration of 20 µg/ml.conclusion: further studies are required to improve solubility of nano-mgo by use of other agents such as peg, and also to confirm our results on other colon and gastric cancer cell lines.