Molecular Diagnostics of Candida Albicanse Secreted Phospholipase B Genes, Yeast, Popular Vaginal Infectious Agent in Women in Tehran City

Background and aim: phospholipases are functional enzymes that are important in the biological process of organisms. these enzymes can hydrolyze glycophospholipids with esterase activity and they invade tissue and have an important role in pathogenesis. candida albicans is one of the most common microorganisms in the human body that affected by phospholipase enzymes. the aim of this study was molecular diagnosis of c. albicans genes plb1 and plb2, and vaginal infection agent in women in tehran citymethods: fifty of c. albicans isolates were collected from patients with vaginitis symptoms who had referred to health centers in tehran city. dna extraction was performed using a dna extraction kit and amplified by pcr for detecting plb genes (plb1 and plb2) with specific primers. the pcr products were sequenced by microsynth company in switzerland and fasta format of sequences were extracted using chromas lite software. then sequence analysis was performed by blast program in ncbi database. dna sequences translated to amino acid sequences using sequence translation tools (ebi, (expasy). the three-dimensional structure and the secondary and estimated protein structure were determined by phyer2 protein fold recognition server.results: results of homology comparison c. albicans gene plb1 shows 88.71% homologous with c. albicans plb1 gene for phospholipase b1 from usa (muzzey et al., 2018). the secondary structure of the protein showed that the protein structure of this gene contains 3% alpha helix and 48% beta strand. maximum phylogenetic relationship with the candida albicans timm 1768. molecular analysis of the plb2 gene shows 88.33% homology similarity with c. albicans plb2 gene for phospholipase b2 from usa (hoover et al., 1998). secondary structure of protein of c. albicans gene plb2 contains 56% alpha helix and 6% beta strand structure. and maximum likelihood (phylogenetic relationship) with the candida albicans sc5314.conclusion: candida albicanse secreted phospholipase b genes (plb1, plb2) polymorphism were found in isolates from patients with vaginitis symptoms who had referred to health centers in tehran.