Crosstalk Between Silicon and Nitric Oxide Mitigates Cu Stress By Up-Regulating of Sod Gene and Antioxidant Enzymes Activities in Salvia Officinalis Plants

Background and aim: copper (cu) contamination is a serious threat to plants and humans. application of silicon (si) or nitric oxide (no) could alleviate cu accumulation and toxicity in plants, but whether they have interactive effects on alleviating of cu accumulation and toxicity are not known. therefore, the combined effect of si and no application on sage (salvia officinalis) growth, cu uptake, regulating of antioxidant enzymes activities and superoxide desmutase (sod) gene were investigated in a pot experiment. here, we reported that cu in toxic concentration decreased growth, caused oxidative stress in plants. the combined application of si and no triggered a significant response in sage, increasing plant growth and reducing oxidative stress symptoms by up-regulating of sod and antioxidant enzymes activities in the plants.methods: real-time pcrresults: increasing in sod gene expression and antioxidant enzymes activities by affecting interactive application of silicon and snp as nitric oxideconclusion: we concluded that combined application of si and no triggered a significant response in sage, increasing plant growth and reducing oxidative stress symptoms by up-regulating of sod and antioxidant enzymes activities in the plants.