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The Effect of Drought Stress on Expression of Genes Coding For Transcription Factors (Zfp252 and Dreb2a) in Rice
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DOR
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20.1001.2.9920068682.1399.1.1.24.8
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نویسنده
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Hamidi Asal ,Tarang Alireza ,Jorjani Eisa
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منبع
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ژنتيك ايران - 1399 - دوره : 16 - شانزدهمین کنگره و چهارمین کنگره بین المللی ژنتیک ایران - کد همایش: 99200-68682
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چکیده
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Drought stress is the most important environmental factor which limits agricultural products in the worlds as well as iran. transcription factors (tfs) can regulate the expression of stress-related genes. in this research, the effect of drought stress in vegetative and reproductive stages on morphological and on expression of genes coding for transcription factors dreb2a and zfp252 was investigated in rice cultivars hashemi (sensitive to drought) and neda (resistance to drought).methods: this experiment was conducted as factorial based on a completely randomized design with three replications. the drought stress was applied through stopping of irrigation at tillering and heading stages.results: the results of variance analysis indicated that there were significant differences between rice cultivars in most of morphological traits. the flag leaf length, flag leaf area and plant fresh weight significantly influenced by drought stress. regarding the tiller number per plant and plant dry weight, there was significant difference between stages which stress applied. the results of real time pcr indicated the increased expression of dreb2a and zfp252 genes in hashemi cultivar under drought stress at vegetative stages, but the expression of zfp252 gene was reduced under drought stress at reproductive stage. in neda cultivar, drought stress at vegetative stage increased expression of zfp252 gene but the expression of dreb2a gene was reduced.conclusion: therefore, our study suggests that these two genes might be involved in drought tolerance of this cultivar to drought stress at the reproductive stage.
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کلیدواژه
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Drought Stress ,Gene Expression ,Rice ,Transcription Factors
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آدرس
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Gonbad Kavous University, Iran, Agricultural Research, Education And Extension Organization (Areeo), Iran, Gonbad Kavous University, Iran
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Authors
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