Purification and Refolding of Recombinant Interferon Alpha-2b Over-Expressed As Inclusion Bodies in Escherichia Coli

Background and aim: interferon alpha 2b is an important cytokine used as a therapeutic agent for hepatitis and cancer treatment. this protein is produced by recombinant dna technology using bacterium escherichia coli as insoluble inclusion bodies. purification and activation of inactive protein from inclusion bodies are challenging. to obtain active and pure ifn α2b from inclusion bodies, an efficient method under optimized conditions should be used for refolding and purification.methods: in this study, overexpression recombinant ifn-a2b in the form of inclusion bodies (ibs) was obtained using the autoinduction culture of recombinant escherichia coli. inclusion bodies were solubilized in 20% sds and dithiothreitol 50 mm in tris buffer (50 mm) at ph 8, then ifn α2b was purified using solvent extraction with 2-butanol as an organic solvent. the purified ifn α2b was refolded in a concentration of 25 µg ml-1 using the dilution method in refolding buffer containing 0.64 mm urea, 5.57 mm cysteine, 1.8 mm cysteine, 1mm edta, 5% glycerol and 50 mm tris with 8 ph. the activity of the refolded sample was determined by measuring the in-vitro bioassay activity (antiviral type).results: finally, the purity and activity of interferon alpha 2b respectively were 90% and 2.1 × 108 of the international unit, which was obtained from refolded protein.conclusion: in the developed method refolded ifn α2b was achieved with high purity and suitable activity, which makes this process suitable for the research and biopharmaceutical industry.