Cytochalasin H Cytotoxic Effect on Human Malignant Glioma Cell Line (U87mg) and Plau and Pcdh10 Genes Expression

Background and aim: glioblastoma is the most and common brain tumors malignant. the treatment of glioblastoma is very difficult, due to extensive tissue surrounding of the brain and the aggressive attack. therefore, the investigation of new therapeutic approaches for this disease is very important. the aim of current study was to provide a proof-of-concept on the mechanism of plau and pcdh10 gene expressions after treatment of malignant human glioma cell line (u87mg) with cytochalasin h.methods: in the present experimental study, we have examined cytochalasin h cytotoxic activities on u87mg cells in vitro for the first time. the cells were cultured and treated with 10-5 - 10-9 m of cytochalasin h for 24, 48 and 72 hours. the assessment of cell viability was carried out by mtt assay at 578 nm. mrna expression changes of plau and pcdh10 were then evaluated by qrt-pcr.results: mtt assay showed that cytochalasin h (10-5 m) inhibited the u87mg cancer cells proliferation after 48 hours. analysis of qrt-pcr showed that the plau expression was significantly decreased in comparison with the control (p<0.05). the expression of pcdh10 also showed a significant increase when compared to the control (p<0.001).conclusion: this study shows the effect of cytochalasin h cytotoxic activities on u87mg cells in a dose- and time-dependent manner and tumor regulatory genes in the cytotoxic effect of cytochalasin h on malignant human glioma cell line.