Bio-Hydrogen Production Enhancement of Synechocystis Sp# Pcc6803 Using Systems Metabolic Engineering Approach

Bio-hydrogen production from microalgae and cyanobacteria has attracted much attention as it is renewable, reliable, and economical# the generation of bio-hydrogen from cyanobacteria can be exciting and promising options for clean energy in the future# advances in metabolic engineering may significantly increase hydrogen production in cyanobacteria# manipulation of the competing metabolic pathways by modulating some key enzymes such as hydrogenase and nitrogenase may seem to be a promising method of incrementing bio-hydrogen# in the present work, the metabolic pathways that have an impact on bidirectional nife hydrogenase which used for producing biological hydrogen in synechocystis sp# pcc 6803 has been investigated# therefore, the reactions such as g3pd2_b, h2ase_syn_f, h2tpp_b, and h2tex_b are recommended to be up-regulated; however, alcd19_f, glyalddr_b, glyk, h2otex_b are good candidates to be down-regulated#