تاثیر تمرین مقاومتی بر بیان ژن های pgc1α و sirt1 و srebp1 در بافت کبد رت ‌های نرویستار چاق

ﯾﮑﯽ از ﻋﻮارض ﻧﺎﺷﯽ از ﭼﺎﻗﯽ، اﺧﺘﻠﺎﻟﺎت ﮐﺒﺪی ﻣﯽﺑﺎﺷﺪ ﮐﻪ ﺑﻪ دﻟﯿﻞ اﻧﺒﺎﺷﺖ ﺗﻮده ﭼﺮﺑﯽ اﺳﺖ. ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺗﺎﺛﯿﺮﺗﻤﺮﯾﻦ ﻣﻘﺎوﻣﺘﯽ ﺑﺮ ﺑﯿﺎن ژنﻫﺎی sirt1 و pgc-1α و srebp1 ﮐﺒﺪی رتﻫﺎی ﻧﺮوﯾﺴﺘﺎرﭼﺎق ﺑﻮد. روش ﺷﻨﺎﺳﯽ: ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، 16 رت وﯾﺴﺘﺎرﻧﺮ ﭼﺎق ﺑﺎ ﺳﻦ ﻫﺸﺖ ﻫﻔﺘﻪ و وزن 327/20±17/44 ﮔﺮم، ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ ﺑﻪ دو ﮔﺮوه؛ ﻣﻘﺎوﻣﺘﯽ (8ﺳﺮ) و ﮐﻨﺘﺮل (8ﺳﺮ) ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. رتﻫﺎی ﮔﺮوه ﺗﺠﺮﺑﯽ ﺑﻪ ﻣﺪت ﻫﺸﺖ ﻫﻔﺘﻪ، ﻫﻔﺘﻪای ﭘﻨﺞ ﺟﻠﺴﻪ ﺗﻤﺮﯾﻨﺎت ﻣﻘﺎوﻣﺘﯽ ﺑﺎ ﺷﺪت 50 ﺗﺎ 120 درﺻﺪوزن ﺑﺪن را اﻧﺠﺎم دادﻧﺪ و ﺑﺮای اﻧﺪازهﮔﯿﺮی ﺑﯿﺎن ژن sirt1 و pgc-1α و srebp1 ﮐﺒﺪی ازروش real time-pcr اﺳﺘﻔﺎده ﺷﺪ. از روش آﻣﺎری ﺗﯽ ﻣﺴﺘﻘﻞ و ﺗﯽ واﺑﺴﺘﻪ ﺟﻬﺖ ﺗﻌﯿﯿﻦ اﺧﺘﻠﺎف ﺑﯿﻦﮔﺮوﻫﯽ و درونﮔﺮوﻫﯽ در ﺳﻄﺢ ﻣﻌﻨﯽداری0/05

the effect of resistance training on the expression of pgc1a, sirt1 and srebp1 genes in liver tissue of obese vistar rats

one of the complications caused by obesity is liver disorders, which is due to theaccumulation of fat mass. the aim of this study was to investigate the effect of resistance trainingon the expression of sirt1, pgc-1α and srebp1 genes in the liver of vistar obese rats.methods: in this study, 16 obese wistar rats, eight weeks old and weighing 327.20±17.44 grams,were randomly divided into two groups; resistance (n=8) and control (n=8) were divided. therats of the experimental group performed five sessions of resistance training with an intensity of50 to 120% of body weight for eight weeks, and real time-pcr method was used to measurethe expression of sirt1, pgc-1α and srebp1 genes in the liver. independent t and dependentt statistical methods were used to determine inter-group and intra-group differences at thesignificance level of p≤0.05.results: the results of the present study showed that resistance training caused a significantincrease in the expression of sirt1 (p=0.001), pgc-1α (p=0.001) and a significant decrease insrebp1 (p=0.001) compared to the control group.conclusion; according to the results of this research, resistance training reduce thecomplications caused by obesity by making changes affecting the liver tissue.