toxic effects of bintaro (cerbera manghas) seed extract on aedes aegypti mosquito

The use of chemicals in disease vector control has had a negative impact on the quality of environmental health. to reduce this impact, this study aimed to evaluate the effect of different bintaro fruit seed extracts (n-hexane and 96% ethanol) on the mortality of ae. aegypti mosquitoes. a total of 1120 samples of 3-5-day-old adult ae. aegypti mosquitoes were used in this study, with 3 repetitions. the research design was experimental, using a post-test-only controlled group design. mosquito mortality was recorded after 24 hours of observation, while the toxicity effect of the spray on adult mosquitoes was recorded at 3-minute intervals for 20 minutes. the results showed that the administration of n-hexane and 96% ethanol extract solutions of c. manghas fruit seeds with different concentrations had different impacts on mosquito mortality. lc50 and lc90 for the n-hexane solution were 11,840 (118,400 ppm) and 26,646 (262,460 ppm), while the 96% ethanol extract solution was 5,207 (52,070 ppm) and 10,436 (104,360 ppm) in 100 ml of distilled water. an anova test on n-hexane and 96% ethanol extracts of c. manghas seeds showed a significant difference between the two types of solutions. the yield of the maceration method using 96% ethanol solvent showed higher results than the n-hexane yield, with a significant value of 0.001 (p < 0.05). there is a significant difference between each concentration of bintaro fruit seed extract and the mortality of ae. aegypti mosquitoes.