asb-16 was superior to sds 0.5% as a decellularization agent: an in vitro study using bovine pericardium

Bovine pericardium offers regenerative medicine potential in applications like heart tissue repair and implantation materials. to utilize it safely, a decellularization process is crucial to remove cells and antigens. this study aims to compare the characteristics of bovine pericardium decellularized using sds 0.5% and asb-16 by evaluating certain parameters. decellularization was performed with sds 0.5% and asb-16. histological analysis, observation using sem, immunohistochemistry, and mtt assay were performed. p < 0.05 was considered significant. there were no nuclei in the bovine pericardium group treated with both methods. a picture of collagen was obtained in sds 0.5%. in the asb-16, collagen was mild (40%), moderate (40%), and dense (20%). in the group decellularized with asb-16, gag was mild (20%), while the other 80% did not show gag. the mean pore size of the bovine pericardium group decellularized with sds was 0.5% smaller than that of asb-16. the differences in type i and type iii collagen intensity were not significant. asb-16 produced significantly more living cells than sds 0.5%. asb-16 has better biomechanical characteristics, namely tensile strength (9.0409 n/m2), tensile strain (1.244 m), and young`s modulus (1.56 n/m2). asb is superior to sds 0.5% as a decellularization agent in bovine pericardium in terms of pore size, cytotoxicity, tensile strength, tensile strain, and young’s modulus. the bovine pericardium scaffold decellularized with asb-16 has a larger pore size, less toxic properties, greater strength or tension, greater tensile strength, and less stiff properties than sds 0.5%.