an antibacterial activity of the produced and purified l-glutamate oxidase from streptomyces sp

From streptomyces species isolated from agricultural soil, an extracellular l-glutamate oxidase was recovered. the streptomyces bacterial isolates were grown in a selective medium that had l-glutamate as a substrate. the filtrate, which is representative of the crude enzyme, was subsequently obtained by extracting the extracellular enzyme using a cooling centrifugation procedure. l-glutamate oxidase purification operations were then performed, starting with fractionation with 40% ammonium salts and continuing with ion-exchange chromatography and gel filtration with a final 8.25 units/mg and 61.8% yield. both positive and negative bacteria were sensitive to the pure l-glutamate oxidase’s effects. purified l-glutamate oxidase outperformed the other examined bacterial isolates in terms of activity, with mic values of 32 µg/ml for klebsiella pneumonia and 64 µg/ml for staphylococcus aureus and escherichia coli. thus, pure l-glutamate oxidase may be viewed as a promising possibility in the rational design of new antibiotics.