fabrication and analysis of nano-hydroxyapatite [ca10(po4)6(oh)2] composites with collagen derived from eggshells through freeze-drying

This study aims to know how to composite hydroxyapatite-collagen by freeze-drying method, and the characterization of hydroxyapatite-collagen composites using xrd, ftir, and sem. hydroxyapatite nanocrystals are synthesized using the precipitation method by reacting cao and (nh4)2hpo4 and the freeze-drying method in composite manufacturing by reacting hydroxyapatite nanocrystals with collagen. the results showed that hydroxyapatite nanocrystals can be composite with collagen using the freeze-drying method, and the results of characterization of xrd, ftir, and sem hydroxyapatite-collagen composites show the formation of chemical bonds between hydroxyapatite and collagen. the xrd spectrum of hap-collagen shows a peak at 2θ 10.45°, showing a physically formed composite. ftir characterization shows the presence of absorption bands po43- and oh- from ha, slight wavenumber shifts also occur in c-h, c-n, n-h, and c=o groups which are characteristic of collagen, this indicates that composites are formed physically. sem characterization shows hap is perfectly deposited into collagen molecules.