antimicrobial effectiveness of wild-type bacillus spp vs bacillus subtilis atcc 6051 against staphylococcus aureus and klebsiella spp isolated from bovine mastitis

Bovine mastitis significantly affects milk production and is a major source of antimicrobial-resistant bacterial agents. the objective of this study is to evaluate the antimicrobial effectiveness of bacillus subtilis atcc 6051 and wild-type bacillus spp. against staphylococcus aureus and klebsiella spp. in bovine mastitis. the study utilized the well diffusion method, three factors were considered such as a (direct diffusion and filtered diffusion methods), b (commercial bacillus subtilis and wild bacillus spp.), and c (klebsiella spp. and staphylococcus aureus). the growth curve analysis was used to assess differences in growth capacity between bacillus subtilis atcc 6051 and wild-type bacillus spp. bacillus subtilis atcc 6051 showed higher inhibition zone measurements against staphylococcus aureus and klebsiella spp. compared to wild-type bacillus spp. direct diffusion method exhibited average inhibition zone measurements of 20.40 mm for both staphylococcus aureus and klebsiella spp. with bacillus subtilis atcc 6051. wild-type bacillus spp. had lower inhibition measurements compared to bacillus subtilis atcc 6051. growth curve analysis indicated that bacillus subtilis atcc 6051 had a higher growth capacity in the exponential phase compared to wild-type bacillus spp.