Estimation of the Total Amounts of Manganese Ions in Some of the Medicinal Plants Leaves Using Flow Injection Technique Combined With Photometric Detection Method

For decades, one of the most required methodologies is to develop a new, simple and sensitive analytical method for the determination of trace amounts of elements in plants. therefore, a spectrophotometric method based on continuous flow injection technique combined photometric detector has been developed and validated for the rapid determination of trace with amounts of manganese ions in plant leaves.  the developed method is based on the converting of mn+2 ions to permanganate using hydrogen peroxide in an alkaline medium (koh).  the coloured violet product was measured using homemade photometric detector at 450 nm. under optimum conditions, a flow rate of 1.8 ml. min, hydrogen peroxide concentration and potassium hydroxide, the linearity of the developed method was in the range of 0.1-36 mg l^-1 with 0.03 mg l^-1 and 1.8% as the detection limits and rsd% respectively. 10 mg is the required weight of the powdered sample for the determination of the manganese. using four different types of medicinal plants (catharanthus roseus, taraxacum officinale, vicia faba and pinus nigra), the developed method was applied successfully to determine the total amounts of total mn+2 in medicinal plants leaves. to ensure the validity of the developed method, the obtained results from the developed method and from those obtained from the reported method were statistically compared using paired t-test at 95% confidence interval. the statistical analysis has shown no significant difference between the methods. therefore, the developed method can be used as an alternative analytical method for the determination of the total amounts of manganese in some of medicinal plants.