antinociceptive effects of paeonia daurica subsp. macrophylla root extracts in mice

This study aimed to assess the antinociceptive activity of extracts and fractions of paeonia daurica subsp. macrophylla in balb/c mice. various doses of hydro-alcoholic extract (he), hexane fraction (f-hexane), methanol (f-meoh), and chloroform (f-chcl3), as well as aqueous extracts (ae), were evaluated by a well-known model, a formalin-induced pain test in mice. all extracts, piroxicam 0.1 mg/kg, and negative control groups were administered 30 minutes before formalin injection. flinching, licking, and biting reflexes were measured as painful factors compared with controls at intervals of 0 to 5 minutes, 0 to 15, and 0 to 60 minutes after formalin injection. the acute oral toxicity test of total ethanolic and aqueous extracts showed no signs of toxic effect up to a dose of 5000 mg/kg. in the formalin test at a time interval of 0 to 5 minutes, there was no significant difference between the results of the study groups. in the range of 0 to 15 minutes, the effect of ae (1 g/kg), he (2, 3 g/kg), and f-hexane (1 g/kg) was significantly higher than the positive control group (p<0.01). in the time interval of 0 to 60 minutes as the total time of the experiment, the effect of ae (0.25 g/kg), ae (0.5, 1 g/kg), he (2, 3 g/kg), f-hexane (1 g/kg) were significantly different than the positive control group. it can be concluded that extract of p. daurica ssp. macrophylla might be helpful in the treatment of pain in humans.