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DNA Methylation of JAK2 Gene in Intestinal Biopsy and Peripheral Blood Samples of Patients with Ulcerative Colitis
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نویسنده
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hanaei sara ,sanati golshid ,sadr maryam ,gharibzadeh safoora ,salmanroghani roham ,salmanroghani hassan ,rezaei nima
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منبع
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immunology and genetics journal - 2021 - دوره : 4 - شماره : 3 - صفحه:155 -160
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چکیده
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Background: comprised of two main subtypes (ulcerative colitis (uc) and crohn), inflammatory bowel disease is caused by an interaction between genetic and environmental factors. as of the important role of innate immunity and jak/stat signaling pathway, the current study was designed to investigate the methylation status jak2 gene in blood and tissue samples of patients with uc. methods: genomic dna was extracted from blood and intestinal biopsy samples of 28 uc patients and 28 controls. after bisulfite dna conversion, real-time quantitative multiplex methylation specific pcr (qm-msp) method was applied in order to assess jak2 promotor methylation status. results: the jak2 promotor in the intestinal biopsy samples was significantly hypermethylated in uc as the mean of unmethylated dna was 1.255±1.865 in the patients group, while it was 1.292±4.726 in control group. conclusion: hypermathylation of jak2 gene may play a part in pathophysiology of uc which could result in gene silencing.
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کلیدواژه
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Ulcerative Colitis; JAK2; Methylation; Epigenetics
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آدرس
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tehran university of medical sciences, research center for immunodeficiencies, children's medical center, Iran. universal scientific education and research network (usern), network of immunity in infection, malignancy and autoimmunity (niima), Iran, universal scientific education and research network (usern), network of immunity in infection, malignancy and autoimmunity (niima), Iran. tehran university of medical sciences, molecular immunology research center, children's medical center, Iran, tehran university of medical sciences, molecular immunology research center, children's medical center, Iran, pasteur institute of iran, research centre for emerging and reemerging infectious diseases, department of epidemiology and biostatistics, Iran, tehran university of medical sciences, school of medicine, Iran, shahid sadoughi university of medical sciences and health services, department of gastrointestinal diseases, Iran, tehran university of medical sciences, research center for immunodeficiencies, children's medical center, school of medicine, department of immunology, iran. universal scientific education and research network (usern), network of immunity in infection, malignancy and autoimmunity (niima), Iran
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Authors
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