kit formulation of active pharmaceutical ingredient d,l-hmpao as a brain perfusion diagnostic system

The purpose of this research study is to prepare exametazime (d,l-hmpao) kit for labeling with 99m-technetium radionuclide as a brain perfusion diagnostic system. firstly, the active pharmaceutical ingredient d,l-hmpao was prepared in two steps with the purity of 99.29 %. its molecular structure was characterized using elemental analysis, fourier-transform infrared spectroscopy (ft-ir), and nuclear magnetic resonance (nmr) technique. in second step, the d,l-hmpao kit was prepared using six different formulations and labeled by technetium-99m radionuclide. the high radiochemical yield was attributed to the high amount of sncl2 and adding phosphate buffer. the animal studies were conducted on the three-month old male wistar rats. the results of the biodistribution studies revealed that, the mean activity in brain of all rats was above 1% id/g. this showed the high isomerism purity of the synthesized compound (d,l-hmpao) and optimization of the suggested formulations.