ionic liquid functionlized on multiwall carbon nanotubes for nickel and lead determination in human serum and urine samples by micro solid-phase extraction

In this study, a novel synthesis adsorbent, 1-(3-aminopropyl)-3- methylimidazolium hexafluorophosphate functionlized on multiwall carbon nanotubes ([apmim][pf6]-mwcnts, il@mwcnts) was used for nickel/lead (ni/pb) extraction and determination by dispersive ionic liquid micro solid-phase extraction (dil-μ-spe) coupled to electrothermal atomic absorption spectrometry (et-aas). after dilution of 20 mg of il@mwcnts in 200 μl of acetone, the mixture was injected to 10 ml of human serum/urine samples at ph of 8.0. after sonication for 5 min, the ni(ii) / pb(ii) were extracted by ionic liquid phase and then centrifuged for 2.5 min. the upper liquid phase set aside and ni(ii) / pb(ii) loaded in adsorbent were backextracted by acidic solution at ph=2-3. finally, the concentration of total nickel and lead was determined by et-aas. by optimizing, the limit of detection, linear range, and enrichment factor for nickel and lead were obtained (0.05 μg l−1; 0.1 μg l−1), (0.2-5.8 μg l−1; 0.4- 30 μg l−1) and 24.7; 5.1, respectively (rsd less than 5%). also, the capacity absorption of il@mwcnts for nickel and lead ions were achieved 149.3 mg g-1 and 162.5 mg g-1, respectively. the dil-μ-spe procedure was validated for nickel and lead extraction by spiking of real samples and icp-ms analyzer.