Sulfamethizole functionalized graphene oxide for invitro separation and determination lead in blood serum of battery manufactories workers by syringe filter-dispersivemicro solid phase extraction

The toxic effect of lead (pb) causes to anemia and iron deficiency in human body. so, the lead determination in blood/serum samples is very important. in this study, a novel adsorbent based on sulfamethizole functionalized on nanographene oxide (c3h10n4o2s2-ngo; smngo) was used for extraction of pb(ii) from human blood, serum and plasma samples in battery manufactories workers by syringe filterdispersive- micro solid phase extraction procedure (sf-d-μ-spe). by procedure, 25 mg of sm-ngo mixed with 10 ml of human blood/ serum or plasma samples and aspirated by 10 ml of syringe tube. after sonication of samples for 5 min, the pb ions adsorbed based on sulfur of sm-ngo adsorbent at ph=6 and the solid phase separated by syringe coupled to millex-fg hydrophobic ptfe membrane (0.2 μm). then, the lead ions were back-extracted from sm-ngo/ ptfe by elution phase with 0.5 ml of nitric acid solution (0.5 m). finally, the concentrations of pb(ii) ions were determined by atom trap flame atomic absorption spectrometry (at-faas) after dilution with dw up to 1 ml. after optimization, the linear range (lr), lod and enrichment factor (ef) for pb ions was obtained, 10-500 μg l^-1, 2.5 μg l^-1 and 9.86, respectively. the validation of procedure was confirmed by electhermal atomic absorption spectrophotometer (et-aas) and certified reference material (nist, crm) in human samples.