synergistic antibacterial activity of plant peptide mbp1 and silver nanoparticles combination on healing of infected wound due to staphylococcus aureus

Background: wound infection is a common problem in hospitals and is typically caused by the antibiotic-resistant staphylococcus aureus, which is a major pathogen for skin and soft tissue infections worldwide. objectives: the aim of this study was to investigate the synergistic antibacterial effect of plant peptide mbp-1 and silver nanoparticles on infected wounds caused by s. aureus. materials and methods: the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of mbp-1 and silver nanoparticles both on their own and in combination form were determined against s. aureus via macrodilution and microdilution methods. the synergistic antibacterial effect of silver nanoparticles and mbp-1 was investigated on infected wounds caused by s. aureus in a mouse model. results: the mic and mbc of mbp-1 were found to be 0.6 and 0.7 mg/ml, respectively. mic and mbc of silver nanoparticles were determined to be 6.25 and 12.5 mg/l, respectively. mic and mbc of the silver nanoparticles and mbp-1 combination were found to be 3.125 mg/ml, 0.5 mg/l; and 6.25 mg/ml, 0.6 mg/l, respectively. the infected wound healed properly after the combined use of mbp-1 and silver nanoparticles. conclusions: the synergistic effect was found on the healing of infected wounds caused by s. aureus by using an mbp-1 and silver nanoparticles combination in a mouse model.