characterization of resistance to aminoglycosides in methicillin-resistant staphylococcus aureus strains isolated from a tertiary care hospital in tehran, iran

Background: methicillin-resistant staphylococcus aureus (mrsa) is one of the most common nosocomial pathogens which can cause a broad spectrum of infections. objectives: the current study aimed to describe the frequency and antibiotic susceptibility patterns of clonal groups of gentamicin- resistant strains of mrsa isolated from a tertiary care hospital in tehran, iran. materials and methods: a total of 301 s. aureus isolates were collected during january to november 2012. all of the isolates were identified at the species level and typed using the phene-plate (php) system. the antibiotic susceptibility patterns of the mrsa strains and the presence of different aminoglycoside resistance genes were determined. results:of the301s.aureusisolates,90 (29.9%)strainswereconfirmedasmrsa,andtheyshowedhighresistancetopenicillin,ciprofloxacin, kanamycin, tobramycin, erythromycin, and tetracycline. on the other hand, 43 of the 90 strains (47.8%) were resistant to gentamicin. aac (6’)-ie + aph (2’’), ant (4’)-ia, aph (3’)-iiia, and ant (6)-ia were detected in 65.6%, 42.2%, 20%, and 47.8% of the gentamicin-resistant strains, respectively. diverse php types consisting of seven common types and four single types were identified among the strains. conclusions: our results illustrated the presence of clonal groups of highly gentamicin-resistant strains of mrsa in hospitals in tehran. the php typing method provided useful information for both clonal dissemination and determining the epidemiological links of the clonal groups of the mrsa strains.