|
|
evaluation of biofilm formation among klebsiella pneumoniae isolates and molecular characterization by eric-pcr
|
|
|
|
|
نویسنده
|
seifi kimia ,kazemian hossein ,heidari hamid ,rezagholizadeh fereshteh ,saee yasaman ,shirvani fariba ,houri hamidreza
|
منبع
|
jundishapur journal of microbiology - 2016 - دوره : 9 - شماره : 1 - صفحه:1 -6
|
چکیده
|
Background: klebsiella pneumoniae is among the most frequently recovered etiologic agents from nosocomial infections. this opportunistic pathogen can generate a thick layer of biofilm as one of its important virulence factors, enabling the bacteria to attach to living or abiotic surfaces, which contributes to drug resistance. objectives: the resistance of biofilm-mediated infections to effective chemotherapy has adverse effects on patient outcomes and survival. therefore, the aim of the present study was to evaluate the biofilm-formation capacity of clinical k. pneumoniae isolates and to perform a molecular characterization using enterobacterial repetitive intergenic consensus-polymerase chain reaction (eric-pcr) to determine the dominant biofilm-producing genotype. patients and methods: in the present study, 94 k. pneumoniae isolates were obtained from two hospitals in tehran, iran. biofilm formation was assayed by a modified procedure, then eric-pcr was carried out. results: the distributions of the clinical specimens used in this study were 61.7% from urine, 18.1% from wounds, 11.7% from sputum, and 8.5% from blood. among these isolates, 33% formed fully established biofilms, 52.1% were categorized as moderately biofilm-producing, 8.5% formed weak biofilms, and 6.4% were non-biofilm-producers. genotyping of k. pneumoniae revealed 31 different eric types. biofilm- formation ability in a special eric type was not observed. conclusions: our results indicated that an enormous proportion of k. pneumoniae isolated from sputum and surgical-wound swabs produced fully established biofilms. it is reasonable to assume the existence of a relationship between the site of infection and the formation of biofilm. a high level of genetic diversity among the k. pneumoniae strains was observed.
|
کلیدواژه
|
biofilm ,pcr ,klebsiella pneumonia
|
آدرس
|
shahid beheshti university of medical sciences, pediatric infections research center, mofid children’s hospital, ایران, tehran university of medical sciences, school of medicine, department of medical microbiology, ایران, shiraz university of medical sciences, school of medicine, department of bacteriology and virology, ایران, tehran university of medical sciences, school of medicine, department of medical microbiology, ایران, islamic azad university, pharmaceutical branch, department of microbiology, ایران, shahid beheshti university of medical sciences, pediatric infections research center, mofid children’s hospital, ایران, shahid beheshti university of medical sciences, school of medicine, department of microbiology, ایران
|
پست الکترونیکی
|
hr.houri@gmail.com
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|