inhibitory effect of bmap-28 on leptospiral lipopolysaccharide-induced tlr2-dependent immune response in bovine cells

Background: bovine leptospirosis is a widespread zoonotic disease, leading to serious economic losses in animal production and causing potential hazards to human health. leptospiral lipopolysaccharide (l-lps) plays an important role in leptospirosis pathogenicity. objectives: with respect to l-lps endotoxin-like activity, we examined bovine immune response to l-lps and the inhibitory ability of bovine myeloid antimicrobial peptide-28 (bmap-28) against l-lps-induced immune activation in bovine cells. materials and methods: in this study, l-lps-induced proinflammatory cytokine production in bovine cells was quantitatively measured with real-time pcr and elisa, and we determined which cell membrane receptors (toll-like receptor [tlr]2 and tlr4) played a major role. in addition, the ability of bmap-28 to inhibit l-lps-induced endotoxin-like immune activation in bovine cells was determined by the decrease in cytokine secretion. results: l-lps showed the ability to induce cytokine production in bovine cells, and its induction was tlr2-dependent. bmap-28 was used to inhibit l-lps-induced endotoxin-like activity. the function of bmap-28 was to inhibit lps-induced tlr2 expression and cytokine production. conclusions: in this study, the l-lps immune response of bovine cells was significant, indicating that tlr2 is the predominant receptor for l-lps. due to l-lps endotoxin-like activity, we found a strategy through using bmap-28 to prevent l-lps-induced tlr2- dependent immune activation in bovine cells.