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   Molecular Identification and Prevalence of Malassezia Species in Pityriasis Versicolor Patients From Kashan, Iran  
   
نویسنده Talaee Rezvan ,Katiraee Farzad ,Ghaderi Maryam ,Erami Mahzad ,Kazemi Alavi Azam ,Nazeri Mehdi
منبع jundishapur journal of microbiology - 2014 - دوره : 7 - شماره : 8 - صفحه:1 -6
چکیده    Background: malassezia species are lipophilic yeasts found on the skin surface of humans and other warm-blooded vertebrates. it is associated with various human diseases, especially pityriasis versicolor, which is a chronic superficial skin disorder.objectives: the aim of the present study was to identify malassezia species isolated from patients’ samples affected by pityriasis versicolor, using molecular methods in kashan, iran.patients and methods: a total of 140 subjects, suspected of having pityriasis versicolor from kashan, were clinically diagnosed and then confirmed by direct microscopic examination. the scraped skin specimens were inoculated in modified dixon’s medium. dna was extracted from the colonies and pcr amplification was carried out for the 26s rdna region. pcr products were used to further restriction fragment length polymorphism by cfoi enzyme.results: direct examination was positive in 93.3% of suspected pityriasis versicolor lesions. no statistically significant difference was observed in the frequency of malassezia species between women and men. the highest prevalence of tinea versicolor was seen in patients 21–30 years-of-age. no difference could be seen in the frequency of malassezia species depending on the age of the patients. in total, 65% of patients with pityriasis versicolor had hyperhidrosis. the most commonly isolated malassezia species in the pityriasis versicolor lesions were; malassezia globosa (66%), m. furfur (26%), m. restricta (3%), m. sympodialis (3%), and m. slooffiae (2%). malassezia species were mainly isolated from the neck and chest.conclusions: this study showed m. globosa to be the most common malassezia species isolated from malassezia skin disorders in kashan, iran. the pcr-rflp method was useful in the rapid identification of the malassezia species. by using these methods, the detection and identification of individual malassezia species from clinical samples was substantially easier.
کلیدواژه Malassezia ,Pityriasis versicolor ,Molecular diagnosis
آدرس kashan university of medical sciences and health services, Dermatology Department, Kashan University of Medical Sciences, Kashan, IR Iran, ایران, university of tabriz, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IR Iran, ایران, kashan university of medical sciences and health services, Dermatology Department, Kashan University of Medical Sciences, Kashan, IR Iran, ایران, kashan university of medical sciences and health services, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, IR Iran, ایران, kashan university of medical sciences and health services, Medical Parasitology and Mycology Department, Kashan University of Medical Sciences, Kashan, IR Iran, ایران, kashan university of medical sciences and health services, Medical Parasitology and Mycology Department, Kashan University of Medical Sciences, Kashan, IR Iran, ایران
پست الکترونیکی mehdinazeri@yahoo.com
 
     
   
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