Study of Polyols Production by Yarrowia lipolytica in Batch Culture and Optimization of Growth Condition for Maximum Production

Background: sugar alcohol erythritol is a non-caloric sweetener, non-cariogenic, andsafe for diabetics because of no change to blood glucose and insulin levels after oraladministration. erythritol cannot be degraded by any enzymatic systems and must beeliminated from the blood through the kidney.objectives: the aim of this study was production and optimization of erythritol fromglucose by yarrowia lipolytica.materials and methods: y. lipolytica dsm70562 was cultivated at 30°c in a 250 ml erlenmeyercontaining 50 ml of production medium composed of 200 g/l glucose, 10 g/lyeast extract, 10 mg/l mnso4.4h2o, and 2 mg/l cuso4.5h2o. erythritol was separatedfrom the sugars and other polyols by thin layer chromatography. total polyols was determinedusing colorimetric method of bok and demain, and erythritol was also elutedfrom the paper and determined by this colorimetric method.results: in a batch culture with 200 g/l glucose at ph 5.5, an erythritol producer of y.lipolytica capable to produce 27.8 g/l erythritol after seven days was selected, correspondingto a 21.52% yield and a productivity of 0.165 g/l/h.conclusions: in this investigation we optimized the production medium and throughaltering medium components that resulted in a drastic change in polyol composition.present study reports the production of erythritol for the first time by a y. lipolytica straindsm70562. due to increasing demand for erythritol as a low caloric sweetener in foodindustry, its production via biological processes is becoming increasingly important.